rProtein Services Questionnaire

Your Information
First tell us a little about yourself.
*First Name: *Last Name:
*Email: *Phone:
*Company/Institute:
Protein of Interest
Help us understand the protein you are working with.
Protein name or database ID:
Modifications to a.a. sequence:
Affinity / other tag:His    Halotag    GST    FLAG    Other:
Tag removal:      Protease:
List any important structural or functional characteristics (post-translational modification, activity, etc.):

Expression (1 of 2)
What expression system(s) would you like?
System
E. coli     Insect     Mammalian     Other:

Expressed protein is
Soluble     Insoluble     Intracellular     Secreted

Additional information
Expression vector: Strain or cell line:
Expression level: Expression scale:
Expression (2 of 2)
 
Has the protein of interest been successfully expressed in the system(s) indicated on the previous page?

Please check any of the following that might apply or would be required:
Project starts with:
gene synthesis     recombinant plasmid     expression-ready plasmid or virus     glycerol stock

Reproduce your protocol* Expression optimization
Reproduce a literature method* Expression scale up
Other (please describe):
* Please provide detailed protocols or literature references
Purification (1 of 3)
 
Has the protein of interest as described here been successfully purified?

Please check any of the following that might apply or would be required:
Purification starting material will be: produced or procured by Aldevron    provided by Client
Reproduce your protocol* Purification method development
Reproduce a literature method* Purification scale up
Other (please describe):
* Please provide detailed protocols or literature references
Purification (2 of 3)
 
What are your project objectives?
Purity level: Yield:
Concentration: Storage buffer:
Deliver by:
Shipping conditions: Storage conditions:
Purification (3 of 3)
 
What types of analysis are needed?
Western blot Activity assay Analytical SEC
Silver stain Endotoxin MW by MS
Other: